陈素明课题组（质谱研究组）

Autoflex maX - MALDI-TOF MS

生产商: Bruker Daltonics 生产年份: 2018

From routine QC to discovery and characterization, the autoflex maX MALDI-TOF and TOF/TOF systems offer versatility and reliability for the rapid analysis of challenging sample types. With a new 10 bit digitizer providing increased dynamic range, its analytical utility is maximized for detailed profiling of polymers, peptides, proteins, lipids, and glycans with minimal sample preparation. The autoflex maX MALDI systems are built with our proven technological innovations and designed to be a powerful tool for scientists, analytical chemists, and researchers around the world. Since the launch of our first commercially available MALDI-TOF MS system, the REFLEX, in 1992, we have continually worked to improve the analytical performance and capabilities of our flex family of MALDI TOF and TOF/TOF instruments. The early adopters of MALDI technology might never have imagined that enzymatic digests for identification via Peptide Mass Fingerprinting might lead way to LC-free, turn-key identity screens for the QC of complex biotherapeutics, with clear results (using our Biopharma Compass software) in less than 15 minutes, or that profiling of glycan pools can be used to rapidly visualize different mAb batches. Sequence fidelity can be confirmed in a flash with MS/MS analyses, and the low sample consumption of the smartbeam II laser of the autoflex maX supports analytical depth, even for very complex samples.

LTQ XL™ Linear Ion Trap MS

生产商: Thermo Fisher 型号: LTQ XL 生产年份: 2018

Obtain more structural information for proteomics and metabolism applications. The Thermo Scientific™ LTQ XL™ ion trap mass spectrometer offers multiple dissociation techniques—PQD, ETD and CID—for routine structural elucidation. PQD is a proprietary technique that eliminates low mass cut-off concerns. The result is more extensive coverage for predicted and unpredicted metabolites, and the ability to perform peptide quantification using isobaric labels.

Orbitrap Elite™ Hybrid Ion Trap-Orbitrap Mass Spectrometer

生产商: Thermo Fisher 生产年份: 2018 手册:

The Thermo Scientific Orbitrap Elite hybrid mass spectrometer raises the bar for MS and MSn performance and versatility by combining our premier dual-pressure linear ion trap mass spectrometer with a novel high-field Orbitrap™ mass analyzer. The system delivers greater than 240,000 resolution (FWHM), increased sensitivity, high scan speeds and a larger dynamic range.

TIMS Pro Ion Mobility Mass Spectrometer

生产商: Bruker Daltonics 型号: TIMS Pro 生产年份: 2019 手册:

Mass spectrometry (MS)-based proteomics has become a powerful technology for the identification and quantification of thousands of proteins. However, the coverage of complete proteomes is still very challenging due to the limited speed, sensitivity and resolution of current mass spectrometers. The timsTOF Pro uses the Parallel Accumulation Serial Fragmentation (PASEF) acquisition method to provide extremely high speed and sensitivity to reach new depths in shotgun proteomics and phosphoproteomics, using low sample amounts. Achieve near 100% duty cycle for shotgun proteomics A near 100% duty cycle for high sensitivity, high speed shotgun proteomics can now be achieved with the dual TIMS technology. The novel design allows for ions to be accumulated in the front section, while ions in the rear section are sequentially released depending on their ion mobility. This process is called Parallel Accumulation Serial Fragmentation, or PASEF. PASEF: the perfect fit for shotgun proteomics The timsTOF Pro powered by PASEF offers sequencing speed > 100 Hz without losing sensitivity or resolution. This is achieved by synchronizing the quadrupole isolation mass window with the elution time of the specific peptide packages from the TIMS funnel. Superior robustness, no cleaning required Many MS instruments used for shotgun proteomics require biweekly or monthly cleaning when run 24 hours a day on large sample cohorts, and performance degradation is noticeable over even shorter time periods. The superior robustness of the timsTOF Pro means that the instrument can be run 24/7 over many days ot weeks without noticeable loss of sensitivity or other performance metrics.

类别

Autoflex maX - MALDI-TOF MS

LTQ XL™ Linear Ion Trap MS

Orbitrap Elite™ Hybrid Ion Trap-Orbitrap Mass Spectrometer

TIMS Pro Ion Mobility Mass Spectrometer