季哲剑教授课题组

简介 蛋白质复合物科学技术

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Research

ATP is an essential energy currency in our cells, just like electricity to our home. We use a wide variety of electrical appliances in our daily life. Similarly, our cells employ various ATPases, which utilize energy from ATP hydrolysis to remodel their substrate molecules. The AAA-family ATPases are powerful nano-scale molecular machines that can remodel macromolecules, such as proteins and DNA. Their functions range from protein translocation and degradation, to complex assembly and disassembly.

Abstract

In general, a AAA ATPase acts in four stages - substrate recruitment, polypeptide initiation, polypeptide unfolding, and substrate release. Based on their substrate spectra, AAA ATPases can also be divided into three categories – promiscuous type, specific type, and orphan type. Zhejian's previous work has focused on the p97/Cdc48 ATPase, a typical promiscuous-type ATPase, and has uncovered its cryo-EM structures and molecular mechanism when processing a polyubiquitinated protein sub...

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