于浩然课题组

Enhancing thermostability of lipase from <i>Pseudomonas alcaligenes</i> for producing <scp>l</scp>-menthol by the CREATE strategy

2022

期刊 Catalysis Science & Technology

作者 Zhonglang Yu · Haoran Yu · Jinling Xu · Zhe Wang · Ziyuan Wang · Tingting Kang · Kaitong Chen · Zhongji Pu · Jianping Wu · Lirong Yang · Gang Xu

Enhancing the thermostability of D-allulose 3-epimerase from Clostridium cellulolyticum H10 via a dual-enzyme screening system

2022

期刊 Enzyme and Microbial Technology

作者 Yun Feng · Zhongji Pu · Li Zhu · Mianbin Wu · Lirong Yang · Haoran Yu · Jianping Lin

Hot spots-making directed evolution easier

2022

期刊 Biotechnology Advances

作者 Haoran Yu · Shuang Ma · Yiwen Li · Paul A. Dalby

Sequence and Structure-Guided Engineering of Urethanase from <i>Agrobacterium tumefaciens</i> d3 for Improved Catalytic Activity

2022

期刊 Journal of Agricultural and Food Chemistry

作者 Xiumiao Yao · Tingting Kang · Zhongji Pu · Tao Zhang · Jianping Lin · Lirong Yang · Haoran Yu · Mianbin Wu

Substrate access path-guided engineering of L-threonine aldolase for improving diastereoselectivity

2022

期刊 Chemical Communications

作者 Wenlong Zheng · Zhongji Pu · Lanxin Xiao · Gang Xu · Li-Rong Yang · Haoran Yu · Jianping Wu

Computational design of highly stable and soluble alcohol dehydrogenase for NADPH regeneration

2021

期刊 Bioresources and Bioprocessing

作者 Jinling Xu · Haisheng Zhou · Haoran Yu · Tong Deng · Ziyuan Wang · Hongyu Zhang · Jianping Wu · Lirong Yang

Directed Evolution of l-Threonine Aldolase for the Diastereoselective Synthesis of β-Hydroxy-α-amino Acids

2021

期刊 ACS Catalysis

作者 Wenlong Zheng · Haoran Yu · Sai Fang · Kaitong Chen · Zhe Wang · Xiuli Cheng · Gang Xu · Lirong Yang · Jianping Wu

Site‐specifically Incorporate Non‐Canonical Amino Acids into Pseudomonas alcaligenes Lipase to Hydrolyze L‐ menthol propionate among the Eight Isomers

2021

期刊 ChemCatChem

作者 Zhonglang Yu · Haoran Yu · Haibin Tang · Zhe Wang · Jianping Wu · Lirong Yang · Gang Xu

A beginner's guide to molecular dynamics simulations and the identification of cross-correlation networks for enzyme engineering

2020

书籍 Enzyme Engineering and Evolution: General Methods 有全文

作者 Haoran Yu · Paul A. Dalby

Computational Design To Reduce Conformational Flexibility and Aggregation Rates of an Antibody Fab Fragment

2020

其它

Engineering transketolase to accept both unnatural donor and acceptor substrates and produce α‐hydroxyketones

2019

期刊 The FEBS Journal

作者 Haoran Yu · Roberto Icken Hernández López · David Steadman · Daniel Méndez‐Sánchez · Sally Higson · Armando Cázares‐Körner · Tom D. Sheppard · John M. Ward · Helen C. Hailes · Paul A. Dalby

Coupled molecular dynamics mediate long- and short-range epistasis between mutations that affect stability and aggregation kinetics

2018

期刊 Proceedings of the National Academy of Sciences

作者 Haoran Yu · Paul A. Dalby

Enhanced trypsin thermostability in Pichia pastoris through truncating the flexible region

2018

期刊 Microbial Cell Factories

作者 Lin Liu · Haoran Yu · Kun Du · Zhiyan Wang · Yiru Gan · He Huang

Exploiting correlated molecular-dynamics networks to counteract enzyme activity–stability trade-off

2018

期刊 Proceedings of the National Academy of Sciences

作者 Haoran Yu · Paul A. Dalby

Two strategies to engineer flexible loops for improved enzyme thermostability

2017

期刊 Scientific Reports

作者 Haoran Yu · Yihan Yan · Cheng Zhang · Paul A. Dalby

A Novel Strategy for Thermostability Improvement of Trypsin Based on N-Glycosylation within the ��-Loop Region

2016

期刊 Journal of Microbiology and Biotechnology

作者 Chao Guo · Ye Liu · Haoran Yu · Kun Du · Yiru Gan · He Huang

The role of proline substitutions within flexible regions on thermostability of luciferase

2015

期刊 Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

作者 Haoran Yu · Yang Zhao · Chao Guo · Yiru Gan · He Huang

Engineering proteins for thermostability through rigidifying flexible sites

2014

期刊 Biotechnology Advances

作者 Haoran Yu · He Huang

Soluble Expression of Active Recombinant Firefly Luciferase in Escherichia coli

2013

期刊 Journal of Pure and Applied Microbiology

作者 Haoran Yu · Yanjie Liu · Liujiehuo · Xinya Liang · Guangbo Kang · He Huang

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Enhancing thermostability of lipase from <i>Pseudomonas alcaligenes</i> for producing <scp>l</scp>-menthol by the CREATE strategy

Enhancing the thermostability of D-allulose 3-epimerase from Clostridium cellulolyticum H10 via a dual-enzyme screening system

Hot spots-making directed evolution easier

Sequence and Structure-Guided Engineering of Urethanase from <i>Agrobacterium tumefaciens</i> d3 for Improved Catalytic Activity

Substrate access path-guided engineering of L-threonine aldolase for improving diastereoselectivity

Computational design of highly stable and soluble alcohol dehydrogenase for NADPH regeneration

Directed Evolution of l-Threonine Aldolase for the Diastereoselective Synthesis of β-Hydroxy-α-amino Acids

Site‐specifically Incorporate Non‐Canonical Amino Acids into Pseudomonas alcaligenes Lipase to Hydrolyze L‐ menthol propionate among the Eight Isomers

A beginner's guide to molecular dynamics simulations and the identification of cross-correlation networks for enzyme engineering

Computational Design To Reduce Conformational Flexibility and Aggregation Rates of an Antibody Fab Fragment

Engineering transketolase to accept both unnatural donor and acceptor substrates and produce α‐hydroxyketones

Coupled molecular dynamics mediate long- and short-range epistasis between mutations that affect stability and aggregation kinetics

Enhanced trypsin thermostability in Pichia pastoris through truncating the flexible region

Exploiting correlated molecular-dynamics networks to counteract enzyme activity–stability trade-off

Two strategies to engineer flexible loops for improved enzyme thermostability

A Novel Strategy for Thermostability Improvement of Trypsin Based on N-Glycosylation within the ��-Loop Region

The role of proline substitutions within flexible regions on thermostability of luciferase

Engineering proteins for thermostability through rigidifying flexible sites

Soluble Expression of Active Recombinant Firefly Luciferase in Escherichia coli